Figure 1. Posterior enrichment of GFP::PIE-1 does not involve degradation. Zygotes expressing GFP::PIE-1 under the
pie-1 promoter and 3'-UTR (a) and the corresponding fluorescence intensity levels in distinct regions of the zygote (b; pronuclear meeting is denoted as t = 0 and n = 6). The increase in posterior fluorescence intensity is greater in magnitude than the decrease in anterior fluorescence intensity caused by the difference in the volumes of these two regions. (c) The presence of the PIE-1 ORF in GFP::PIE-1 (n = 6) does not affect overall levels of the protein product as compared with GFP alone (n = 6).
mex-5/6(RNAi) prevents the posterior enrichment of GFP::PIE-1 in the zygote (Fig. S1, available at
http://www.jcb.org/cgi/content/full/jcb.200809077/DC1), but does not affect overall protein levels in the zygote. Error bars represent SEM. Bar, 10 um.