(A-D) In the wild-type intestine, GFP-UNC-64 and GFP-SYN-1 localize to the apical and basolateral PM, respectively. In the
snap-29(RNAi) animals, GFP-UNC-64 and GFP-SYN-1 targeted to the PM are reduced and the GFP signals are observed in fine-vesicular cytoplasmic structures. Autofluorescence of lysosome-like organelles can be seen in both the green and red channels, whereas GFP appears only in the green channel. Merged images of green and red channels are shown in A-D, in which autofluorescence is seen as yellow. An enlarged (2) image of the boxed area is shown in the inset (B and D). (A', B') Nomarski images corresponding to A and B, respectively. The wild-type intestine is filled with granules (A', arrowheads). In the intestine of
snap-29(RNAi) animals (B'), there are fewer granules (arrowheads) and the cytoplasm appears grainy (arrows). The lumen of the intestine accumulates undigested E. coli (asterisk).