Fig. 2. GFP reporter expression. (A) A schematic diagram of cell positions in the mid L4 stage and the young adult (Sharma-Kishore et al., 1999; Sulston andHorvitz, 1977). Nuclei are indicated by circles, and prominent cell boundaries are indicated by thin lines. Ventral is down in all panels, and the dark horizontalline represents the ventral cuticle. Since animals were typically observed from the side, different focal planes correspond to the midline (top panel), the sublateralplane (middle panel) and the lateral plane (bottom panel). A three-dimensional schematic is shown to the right. 'A, B1, B2' correpond to 'vulA,vulB1, vulB'. (B) GFP expression. Each set of six panels shows three Nomarski images to the left, and corresponding epifluorescence images to the right.The top panels are from the midline, the middle panels are from the sub-lateral plane, and the bottom panels are from the lateral plane. Strains and arraysphotographed are: NH2246 ayIs4[
egl-17::gfp], PS3528 syIs51[
cdh-3::cfp]; syIs55[
ceh-2::yfp], PS3239 syIs49[
zmp-1::gfp], PS3526 syIs60[F47B8.6::gfp],PS3664 syIs65[B0034.1::
pes-10::gfp] and PS3722 syIs101[T04B2.6::yfp].