Picture from Burmeister C et al. (2008) FASEB J "Oxidative stress in Caenorhabditis elegans: protective effects of the Omega ...."
Figure 4. Analysis of the expression pattern of GSTO-1 in C. elegans. A) Typical expression of GSTO-1::GFP reporter constructs. Weak GFP signals were detected exclusively in the intestinal cells of L1-L4 and adult hermaphrodites. B) Effect of deletion constructs and mutations on GFP expression in gut cells. Worms were microinjected with the constructs GFP-1321, GFP-397, GFP-325, GFP-254, and GFP-178. Fluorescence was observed in transgenic worms expressing the constructs GFP-397 and GFP-325 (as indicated in green). No signal was obtained for the shorter constructs GFP-254 and GFP-178. To investigate the involvement of the GATA element 259-265 bp upstream of the initiation codon (as indicated in red) in regulating transcription, site-directed mutagenesis was used. Mutation resulted in complete loss of GFP expression. C) Immunogold electron microscopy. Part of a cross section of wild-type worms using preimmune (A) and anti-GSTO-1 antibody (B). LD = lipid droplet; MT = mitochondria; IL = inner lumen of intestine; GP = gold particle. Electron micrographs were taken on a Phillips CM-10 transmission electron microscope.