Figure 4. Embryonic ALG-1 and ALG-2 expression. The expression onset of GFP::ALG-2 and RFP::ALG-1 differs. RFP::ALG-1 fluorescence is first detected at the beginning of the morphogenetic phase (B). Scale bar 20 um.
Figure 3. EHBP-1 colocalizes with RAB-10 and -8 on endosomes. All images are from deconvolved 3D image stacks acquired in intact living animals expressing GFP- and RFP-tagged proteins specifically in intestinal epithelial cells. (A-A'') Arrowheads, endosomes labeled by both EHBP-1-GFP and RFP-RAB-5. (B-B'') Arrowheads, endosomes labeled by both EHBP-1-GFP and RFP-RAB-10. (C-C'') Arrowheads, endosomes labeled by both EHBP-1-GFP and RFP-RAB-8. (D-D'') Arrowheads, endosomes labeled by both EHBP-1-GFP and RFP-RME-1. (E-E'') Arrowheads, EHBP-1-GFP-positive endosomes juxtaposed to RFP-RAB-11-labeled structures. (F-F'') EHBP-1-GFP does not colocalize with Golgi marker Mannosidase-RFP. In each image autofluorescent lysosome-like organelles can be seen in all three channels with the strongest signal in blue, whereas GFP appears only in the green channel and RFP only in the red channel. Signals observed in the green or red channels that do not overlap with signals in the blue channel are considered bone fide GFP or RFP signals, respectively. Scale bar, 10 um.