Figure5. Temporal and Sex-Specific Regulation of
xol-1 Transcript Levels An autoradiograph of a Northern blot made from poly(A)+ RNA (25 ug per lane) isolated from either wild-type(wt)(99.8% XX) or
him-8 (37% XO and 63% XX) animals at different developmental stages. The blot was hybridized with labeled RNA generated from a cDNA of the 2.2 kb transcript.The staged populations include early embryos, the oldest of which were at comma stage(6 hr of development) and the majority of which had only completed the first third of embryogenesis; mixed-stage embryos; and L1, L2, and L3 larvae. As a control for consistency of loading and transfer,were probed the blot with both an actin probe specific for
act-1 and a myoD probe that is expressed during early embryonic development (data not shown).Because the
him-8 populations only contain 37% XO animals, the real difference in transcript levels betweenXX and XO animals is 2.5 times the apparent difference.