Figure 2. SAX-7 and MNR-1 Functioned in the Hypodermal Cell (A)
sax-7(
nj48) mutants had severely defective PVD morphology. (B) The mutant phenotype could be fully rescued by expressing SAX-7 in the hypodermal cells using the
dpy-7 promoter. (C and D) SAX-7 was expressed in neurons and hypodermal cells (D) and localized subcellularly to two lateral stripes in the hypodermal cell (C). In (C), arrows indicate SAX-7 sublateral stripes in hypodermal cells, and arrowheads indicate SAX-7 expressed in the ALM and PLM neurons. Arrowhead in (D) indicates fluorescent labeling of the hypodermal cells. (E and F) The PVD morphology defects in mnr- 1
(wy758) (E) were also fully rescued by expressing MNR-1 in the hypodermal cell using the
dpy-7 promoter (F). (G and H) MNR-1 was only expressed in the hypodermis (H) and showed no obvious subcellular localization (G). Arrowhead in (H) indicates fluorescent labeling of the hypodermal cells. (I) Expression of SAX-7 in hypodermal cells rescued the mutant phenotype in all transgenic animals, whereas no rescue was observed when SAX-7 was expressed in the PVD neuron using the
ser2prom3 promoter, or in muscles using the
hlh-1 promoter. (J) Expression of MNR-1 in the hypodermal cells also rescued the
mnr-1(
wy758) mutant phenotype, whereas expression in PVD using the
ser2prom3 promoter failed to rescue it. (K) PVD morphology in
sax-7(
nj48);
mnr-1(
wy758) double mutants was indistinguishable from that in the single mutants. Scale bar, 10 mm. Error bars, SEM. ***p < 0.001 by Student's t test. n = 20 for all genotypes.