Fig 1. TRP-1 and TRP-2 are required for head steering locomotion. (A) Schematic of the SMD neurons in C. elegans and the values analyzed duringforward movement. (B) A phylogenetic tree of the TRPC subfamily channels. (C) The genomic locations (above) and structures (below) of the
trp-1 andtrp-2 genes. The gray lines indicate the regions deleted in the
trp-1 (
sy690) and
trp-2 (
sy691) mutants. Ankyrin repeats, transmembrane domains, andTRP domains are labeled in blue, yellow, and green, respectively. (D) Sinusoidal waveform tracks of wild-type animals (left) and
trp-1 trp-2 double mutant animals (right) on a bacterial lawn. Scale bar: 25 um. (E) Heat maps and a color-coded average turning angle for the indicated genotypes. n = 20for each genotype. (F) Expression patterns of the indicated transgenes. Images in the upper left and lower left boxed regions are single-focal-planeconfocal microscopy images of the SMDV and SMDD soma, respectively. Anterior is to the left. Scale bar: 25 um. (G) Heat maps and the averageturning angle for the indicated genotypes. n = 20 for each. Numerical values that underlie the graph are shown in S1 Data. Error bars indicate SEM.and indicate a significant difference from wild type at p < 0.05 and p < 0.001, respectively (one-way ANOVA test followed by the Tukey post hoctest). TRP, transient receptor potential; TRPC, transient receptor protein cation; VNC, ventral nerve cord