Picture from Hu J et al. (2007) Mol Biol Cell "STAM and Hrs down-regulate ciliary TRP receptors."
Figure 3. STAM-1A is an early endosomal protein that localizes with RAB-5 and HGRS-1 in male-specific neurons. (A) Confocal micrographs of Ppkd-2::STAM-1A:: DsRed2 and Ppkd-2::HGRS-1::GFP double-labeled males. STAM-1A and HGRS-1 colocalize in cell bodies, the bases of cilia, and along RnB dendrites. Like STAM-1, HGRS-1 does not localize to the cilium proper. Arrows point to colocalization of STAM-1A and HGRS-1 at the ciliary base of CEM and R3B. (B) Confocal micrographs of Ppkd-2::STAM-1A::GFP and Ppkd-2::mRFP::RAB-5 double-labeled males. RAB-5 is an early endosomal marker (Sato et al., 2005). STAM-1A colocalizes with RAB-5 in the cell body and ciliary base. (C) Higher magnification images show that RAB-5 and STAM-1A collocate in CEM cell body. (D) Higher magnification fluorescence and DIC/fluorescence images of CEM cilia. RAB-5 and STAM-1A are never observed in the cilium proper. Scale bars, (A and B) 10 um, and (C and D) 5 um.