(A-F) Confocal images of UNC-104::mCherry in the dorsal and ventral nerve cord, which are labeled with myrGFP (D-F), in young (A and D) andaged (B, C, E, and F) worms.
Figure 1. A) General organization of SapTrap-assembled constructs. Italics indicate the fragments that are assembled to build the final construct, and blue upper-case letters are the 3-nucleotide overlaps that enable ordered assembly. B) Efficiency of construct assembly (% correct clones) using SapTrap (Schwartz and Jorgensen 2016) or a ccdB/Gibson-based approach (Dickinson et al. 2015).
(D-F) Wild-type anaphase one-cell stage embryos stained with anti-tubulin (D) and anti-ZYG-8 (F) antibodies. (D)-(F) are at the same magnification. Bar = 10 um. Anti-ZYG-8 antibodies mark the spindle and spindle poles (arrowheads) in wild-type.
(A-F) Expression pattern of the autophagy genes atg-9 (A-C) and atg-2 (D-F) using transcriptional fusions (see Supplemental Experimental Procedures). AIY is identified with cytoplasmic mCh (B and E). (C and F) Mergeimages for (A-B) and (D-E), respectively.
Expression of MBOA-7::GFP in an early embryo (D and E) and a second-stage larva (F and G). Nomarski micrographs (D and F) and corresponding MBOA-7::GFP expression (E and G). MBOA-7::GFP was ubiquitously expressed throughout development. Asterisks indicate the anterior of the larva (F and G). Bar, 10 um (D and E) and 200 um (F and G).
(D-F) The epg-3 reporter is expressed ubiquitously in embryos (E) and widely in larvae (F). EPG-3 is cytoplasmic. (D) Nomarski image of the embryo in E.