Figure 1. Characterization and analysis of
mat-3 alleles:(A) DAPI and DIC images of wildtype,
mat-3(
as49), and
mat-3(
or344) hermaphrodites. Arrowheads indicate the increased number of embryos where the sperm and oocyte DNA fail to proceed through meiosis. Scale bar equals 10mm. (B) Brood size of
mat-3(
as49), and
mat-3(
or344) hermaphrodites at 16°C (n>=9, one-way ANOVA, ****p<0.0001). (C) Brood size of
mat-3(
as49), and
mat-3(
or344) hermaphrodites at 25°C (n>=10, one-way ANOVA, ****p<0.0001). (D) Genomic locus of
mat-3 indicating the mutations that generated
mat-3(
as49) and
mat-3(
or344). Red boxes indicate where the TPR domains are encoded. Each blue box indicates an exon. (E-F) Sequences of TPR domains 6 and 7. Red boxes indicate amino acids that were mutated and the change in DNA sequences are reflected in parentheses.