Figure 1. Identification of mutations in eIF1. (A) Chromosomal location of
eif-1(
nb132). (B) GFP expression from extrachromosomal arrays coexpressing GUG-GFP reporter and the
eif-1(
nb132) (C65G) mutant eIF1. (C) eIF1 is an alternatively spliced open reading frame. T27F7.3 (exons 1, 2, 3, 4a, and 5 with blue lines indicating splicing) contains the continuous coding region of eIF1. T27F7.4 (exons 1, 2, 3, 4, and 5 with black lines indicating splicing) contains the coding region of mannosyltransferase III (shown as shaded region in exon 4), which interrupts the coding region of eIF1. GFP fusion constructs for examining the expression of the two open reading frames are shown at the bottom. (D) Fluorescent images (left) and corresponding DIC images (right) of worms expressing GFP-fusion transgenes. Arrowhead, GFP expression in the pharynx; arrow, expression in the intestine.