Figure 3. Expression pattern of KCNL-2. A. The
kcnl-2 gene was amplified from the WRM063DE08 fosmid and GFP was fused to the latter stop codon to produce a translational gene fusion that tagged the channel at the carboxy terminus. pkcnl-2kcnl-2
(taa2)::gfp localized to the neurons of the head, the NR, motor neurons of the VNC, the DC, the pharyngeal nervous system, and to tail ganglia (widefield fluorescent image of strain VK1567). B-G. Magnified confocal images of pkcnl-2kcnl-2
(taa2)::gfp (B-D) and pkcnl-2gfp ::(
atg2)
kcnl-2 (E-G; strain VK1401) when expressed in the N2 background (Scale bar=10 μm). B,E. Neurons of the head, the pharyngeal nervous system, and cell bodies of the nerve ring. C. Neuronal processes innervating the vulva. F. Neuronal processes at the vulva, including the VC4 & VC5 cell bodies (arrows). D,G. Tail ganglia.