Corresponding Nomarski (bottom) and epifluorescence (top) images are shown. The anchor cell is indicated by large arrowheads. Identities of VPC descendants are indicated below. Scale bar = 20 μm.(A) Full-length (C-term) LIN-18::GFP fusion under the control of the
lin-18 promoter. Expression in P5.p and P7.p granddaughters is shown. Although some puncta of GFP fluorescence were observed with this and other fusions, all TM domain-containing GFP fusions were on the plasma membrane.(C) Full-length LIN-18::DsRed2 fusion protein under the control of the
lin-18 promoter (shown in red and membrane localized) and nuclear localized GFP under the control of the
lin-17 promoter. Expression in P7.p daughters is shown. P5.p and P6.p daughters also express
lin-17::gfp and
lin-18::gfp (or DsRed2) (not shown).(D) Membrane localized
lin-18::gfp in VPCs, P6.p and P7.p.