Figure 5. Characterization of W01G7.1/daf-5. (A) Molecular identification of
daf-5 as W01G7.1. The
daf-5(
m14) allele carries a GGC to GAC change resulting in a Gly to Asp substitution at amino acid 219 in exon 4 of W01G7.1. The
m512 allele corresponds to a deletion of W01G7.1.(B-F) Expression pattern of W01G7.1/daf-5 in mEx127.(B) L2 larva showing expression in anterior pharynx and head neurons. ga, gut autofluorescence.(C) L2 larva showing gut autofluorescence and GFP expression in tail neurons.(D and E) Adult expression patterns showing variability in posterior pharyngeal expression.(F) Expression in the adult tail.