Ectopic ASH gene expression in
mls-2(
cc615) mutant animals requires
unc-42.: UNC-42 function is required for the ectopic expression of ASH terminal differentiation gene markers in the ectopic ASH-like cells of
mls-2 loss-of-function animals. The native ASHs and ectopic ASH-like neurons were identified by positional labeling with the lipophilic dye DiD, as previously described (Perkinset al., 1986). (A) Representative images of
sra-6p::gfp are shown. Native ASH neurons of wild-type and
unc-42 animals are indicated by white arrows. Native and ectopic ASH-like cells are indicated for
mls-2 and
unc-42;
mls-2 animals. (B) Pie charts indicate the degree of bilateral ectopic marker expression in
mls-2(
cc615) animals, as well as right versus left marker expression in the cases of unilateral ectopic expression. (C) The percentage of animals with marker expression in 4, 3, 2, 1 and zero (2 off) ASH and ASH-like cells, combined, is shown. n > 34 animals were examined for each genotype.