Figure 1. Outcrossing of a Million Mutation Project strain with the fluorescence balancer tmC18[tmIs1200].(A) Dissecting microscope view of freshly starved wild type worms (top),
gk837385 worms before outcross (VC40832, middle), and
gk837385 worms after outcross (FX31729, bottom). The panel of the
gk837385 original strain (middle) has many unhatched eggs (arrows). The panels of wild type (top) and the outcrossed strain (bottom) have many small larvae (arrowheads). Insets: enlarged images of the boxed area. (B) Genomic loci covered by tmC18. (C) The outcrossing scheme to segregate away unlinked mutations using the fluorescently labeled inversion balancer (shown by green). N2 males are first crossed to the fluorescently labeled inversion balancer strain to generate heterozygous tmC18[tmIs1200]/+ males (P0). Males resulting from this cross are then crossed to the
ego-1 mutant hermaphrodites (F1). Venus-positive hermaphrodites resulting from the second cross are then self-fertilized (F2). Following self-fertilization, a non-Venus hermaphrodite is isolated (F3).