Figure 5. Hyperactive Feeding Requires an NMDA Receptor to Peptidergic Signaling Axis that Converges on a Serotonergic Sensory Neuron(A) Pharyngeal pumping rates of wild-type,
nmr-1, and
nmr-2 mutants cultured on either RNAi vector control or
nkat-1(RNAi). *p < 0.0001, ANOVA (Tukey).(B) Pharyngeal pumping rates at the indicated fasting and refeeding periods. ns, p > 0.1 and *p < 0.0001. ANOVA (Tukey) comparing fasted, post-fast animals toad-libitum-fed controls.(C) Pharyngeal pumping rates of the indicated strains. Animals were cultured on bacteria expressing
nkat-1(RNAi) or RNAi vector control. *p < 0.0001 ANOVA(Sidak) comparing vector control to
nkat-1(RNAi) for each genotype.(D) Pharyngeal pumping rates of wild-type,
flp-18 mutants, and
flp-18 transgenic lines used in (C) fed ad libitum, after 2 hr of fasting, and after 5 min ofpost-fast refeeding. *p < 0.05 and **p < 0.0001. ANOVA (Tukey) comparing fasting and post-fast measurements to that of ad libitum fed for eachmeasurement.(E) Pharyngeal pumping rates of wild-type,
npr-5 mutants, and
npr-5 mutants expressing transgenes directing expression of
npr-5a and -5b to ADF neurons.Animals were cultured on bacteria expressing either RNAi vector control or
nkat-1(RNAi). *p < 0.0001. ANOVA (Sidak) comparing vector control treated tonkat-1(RNAi) treated for each genotype.