Figure S7. Phenotypic studies of wild type and mutant hermaphrodites. (A-C) DTC migration and EFN-2 expression. (A) The DTC migrates along the ventral body wall muscle (blue) between the
hyp7 hypodermal syncytium (green) during phase 1. The DTC then turns and crosses
hyp7 during phase 2. In phase 3, the DTC turns again and migrates along the dorsal body wall muscle. Its final resting position is shown in red. Wild type and mutant hermaphrodites were scored for defects in each phase of DTC migration. (B-C) An
efn-2::GFP transcriptional reporter (Wang et al., 1999) shows GFP expression in
hyp7 (arrowhead) during the time of DTC migration (B), but not before DTC migration (C). The bright spots in panel C are nonspecific gut autofluorescence. These results are consistent with EFN-2 functioning in
hyp7 to repel the DTC, keeping it migrating along the body wall muscle. (D) Embryonic [E] and larval [L] lethality of wild type and mutant hermaphrodites. (E) 4D time-lapse DIC micrographs of wild-type and mutant embryos undergoing ventral enclosure. During enclosure, the ventral hypodermal cells (brown cells, diagram) migrate and meet at the ventral midline. The blue cells and green cells are the seam and dorsal hypodermal cells, respectively.
vpr-1(
tm1411) embryos have incompletely penetrant and variably expressed enclosure defects that are similar to
vab-1(
dx31) null embryos.
vpr-1(
tm1411);
vab-1(
dx31) double mutants exhibit similar defects as the single mutants, although the double mutants exhibit increased embryonic lethality (D).