Figure 3.
fos-1a Is Expressed at High Levels in the AC during Invasion. (A) YFP and CFP insertions into the start
fos-1a and
fos-1b exons, respectively, to create transcriptional (-TX) and translational (-TL) transgene reporters.(B) Nomarski (left) and corresponding fluorescence image show that
fos-1a::YFP-TL fusion protein (right) is localized at high levels in the nucleus of the AC (arrow) and at lower levels in the neighboring uterine cells in the late L3 (P6.p four-cell stage). The 1st-fated vulval cells (bracket) show no FOS-1A::YFP expression.(C) The
fos-1b::CFP-TL fusion protein is expressed at low levels in the AC (arrow) and neighboring uterine cells, as well as the underlying vulval cells.(D) Nomarski image (left),
fos-1a::YFP-TX expression (purple, center), and
fos-1b::CFP-TX expression (green, right) of an entire late-L3 larva show that
fos-1a expression is primarily confined to the somatic gonad (arrow indicates AC location), while
fos-1b is expressed in most cells.The scale bars are 10 μm.