Figure 3. PPTR-1 Colocalizes with AKT-1.
akt-1::gfp;
pptr-1::mC-flag,
akt-2::gfp;
pptr-1::mC-flag, and
sgk-1::gfp;
pptr-1::mC-flag transgenic worms were mounted and visualized by fluorescence microscopy with Rhodamine (mCherry) and FITC (GFP) filters. PPTR-1 expression is observed mainly in the pharynx, vulva, and spermatheca ([A]-[C], mCherry).(A) Expression of PPTR-1::mC-FLAG (mCherry) and AKT-1::GFP (GFP) in a
akt-1::gfp;
pptr-1::mC-flag strain. Expression of PPTR-1::mC-FLAG overlaps with AKT-1::GFP (Merge).(B) PPTR-1::mC-FLAG and AKT-2::GFP colocalize in some tissues in a
akt-2::gfp;
pptr-1::mC-flag strain (Merge).(C) SGK-1::GFP and PPTR-1::mC-FLAG do not colocalize in
sgk-1::gfp;
pptr-1::mC-flag transgenic worms (Merge).Arrows indicate the following tissues: p, pharynx; v, vulva; s, spermatheca; i, intestine.