Figure 4. ZYG-12 Isoforms Localize to Both the Centrosome and Nucleus. Timelapse two-photon images of embryos expressing GFP-tagged ZYG-12 isoforms. Embryos are expressing (A and B) ZYG-12A,B,C; (C and D) ZYG-12A; (E and F) ZYG-12B,C tagged with GFP. (A), (C), and (E) are at the pronuclear migration stage and (B), (D), and (F) are at the centration stage. (C and E) Only one of two centrosomes visible in focal plane. (G) Wild-type embryo at the two cell stage stained with ZYG-12 antibodies. Centrosomes in the anterior cell AB are out of the plane of focus. (H)
tba-2 RNAi embryo expressing ZYG-12A,B,C::GFP. All embryos shown are wild-type for the genomic copy of
zyg-12. For movie corresponding to Figures 4A and 4B, see Supplemental Movie S3 on Cell website. Bar, 10 μm.