Figure 2.
olrn-1 encodes a protein with Raw repeats and potential transmembrane domains. (a) Genomic structure of
olrn-1, showingalternative first exons for a and b isoforms, whose 5' ends are separated by 3.8 kb. (b) Translation of
olrn-1, showing alternativefirst exons for
olrn-1a and
olrn-1b isoforms. Two short repeats shared with Drosophila Raw are highlighted in green withconserved residues in bold type; potential transmembrane domains are boxed. The location of the splice acceptor site (fourthexon) mutated in
ut305 and the residue mutated in
olrn-1(
ky626) (G 473a/466b E) are marked. Arrow marks the carboxy-terminalinsertion site of Cherry in
odr-3::
olrn-1b::Ch (f). (c) Expression of
olrn-1a::Cherry promoter fusion. Arrowhead indicatesCherry expression in AWC neuron expressing
str-2::GFP. (d) Expression of
olrn-1b::Cherry promoter fusion in non-neuronalcells. Arrows, hypodermal cells. The pharynx is a prominent site of expression. Arrowhead, no Cherry expression in AWCneuron expressing
str-2::GFP. (e) Expression of amino-terminally tagged
odr-3::Cherry::
olrn-1b in an L4
olrn-1(
ky626) animal.
odr-3::Cherry::
olrn-1b is excluded from the nucleus and is punctate in the axon and dendrite. (f) Expression of carboxy-terminallytagged
odr-3::
olrn-1b::Cherry in an L4
ky626 animal.
odr-3::
olrn-1b::Cherry is excluded from the nucleus, and is punctate in the axonand dendrite. Arrowheads, AWC cell bodies. Scale bars, 20 um. Images are stacked confocal images.