Figure 1. Endogenous TOP-2 localization using a GFP-tag: (A-F) Live imaging of the
top-2::gfp C. elegans strain using confocal microscopy. (A) A complete representation of TOP-2 localization in the germ line of
top-2::gfp worms. Scale bar= 50 µm. (B, C) TOP-2 localization in the head and tail, respectively, of
top-2::gfp worms. Scale bar= 50 µm (D) TOP-2 localization during pachytene of
top-2::gfp worms. White arrowheads point to examples of TOP-2::GFP foci. Scale bar= 5 µm. (E) TOP-2 localization in -1 and -2 oocytes in
top-2::gfp worms. Scale bar= 5 µm. (F) TOP-2 localization in developing embryos in
top-2::gfp worms. Scale bar= 50 µm. (G) Brood size comparison of endogenously tagged
top-2::gfp to wild type (N2). (H) Percent embryonic viability from the
top-2::gfp strain compared to wild type (N2). Statistics were conducted using a two-tailed Student's T-test, n.s. indicates not significant, *p=0.0001.