Fig 1.
daf-41/ZC395.10 regulates dauer formation and stress resistance. (A) An alignment of protein sequences between C. elegans DAF-41, D. melanogaster CG16817 and Homo sapiens
p23/PTGES3. The similarity between DAF-41 and
p23/PTGES3 is 44.6 percent. (B) Schematic illustration of the
daf41, and deletion alleles of
ok3015 and
ok3052. Black arrows indicate the direction of transcription. Red area indicates HSP20-like co-chaperone domain. (C)
daf-41 mutants constitutively formed dauer larvae (Daf-c) weakly at 25 degrees C and strongly at 27 degrees C. (D) Dauer alae of
daf-41(
ok3052) animals grown at 27 degrees C are indicated by the white arrows. (E)
daf-41(
ok3052) worms were resistant for oxidative stress (20uM of H2O2, 2.5 hrs) and heat stress (35 degrees C, 8 hrs).
gst-4 (
ok2358) worms were also slightly stress tolerant. (F)
daf-41p::gfp (i.e.
dpy-5(
e907); sEx10796 [rCes
daf-41::gfp + pCeh361]) worms were labeled with DiI and photos taken at the young adult stage. Patterns of gene expression of
daf-41p::gfp (green), DiI (red), and merged figures are shown, with arrows indicating individual neurons.