Figure 1.
gtl-2 Acts in Nonneuronal Tissues to Modulate
acr-2(gf) Convulsions (A) Images of wild-type,
acr-2(gf), and gtl- 2(lf);
acr-2(gf) locomotion. (B) Quantification of convulsion frequency in mutants of genotype as indicated. **p < 0.01, compared to
acr-2(gf), ANOVA and Dunnett's post hoc test. (C) Transgenic rescue of the suppression activities in
gtl-2(0);
acr-2(gf) and
gtl-2(lf);
acr-2(gf). *** p < 0.001, ANOVA and Bonferroni post hoc test. (D) Confocal image of anti-GFP immunostaining of GTL-2::GFP in an adult shows expression in the excretory cell (arrowhead) and epidermis (arrow). (E) Confocal image of anti-GFP immunostaining of GTL-2::GFP in a L4 larva expressing Pdpy- 7:gtl-2cDNA::gfp. (F) Cell-specific rescue of
gtl-2 shows requirement in epidermis and excretory cell. Cell types are as follows: Pdpy-30, all cells; Prgef-1, panneuronal; Pmyo-3, body muscles; Psulp-4, excretory cell; and Pdpy-7, epidermis. **p < 0.01, compared to
gtl-2(lf);
acr-2(gf), ANOVA and Dunnett's post hoc test. Error bars indicate SEM.