Figure S2.
lin-65 Is Mainly Expressed in the Intestine and Neurons, Related to Figure 2(A) Representative photomicrograph of
lin-65p::mCherry transgenic animals.
lin-65 is expressed in several head neurons and throughout intestine.(B) Quantitative PCR of
lin-65 mRNA level. Synchronized
day1,
day2 and
day3 adult animals grown on empty vector (EV) or EV+
cco-1 RNAi from hatch werecollected for qPCR. (* denotes p 0.05, ns denotes p > 0.05 via t test, error bar indicates the SEM from three biological replicates).(C) Representative photomicrograph of
lin-65(
n3441);
hsp-6p::gfp;
rgef-1p::Q40::yfp animals with or without
lin-65p::
lin-65::mCherry rescue.(D) Representative photomicrograph of
lin-65p::
lin-65::mCherry transgenic animals.(E) Immunoblots of HA-epitope tagged expression of
lin-65p::
lin-65::HA transgenic animals grown on empty vector, EV+
cco-1 RNAi and
cco-1+
lin-65 RNAi as indicated. Animals were collected at day 1 of adulthood. Anti-tubulin serves as a loading control.