Figure 1.
src-2(Val170Ile) mutant exhibits reduced brood size and enhances embryonic morphogenesis defects in
src-1(RNAi) conditions:(A) Sequence alignment of the conserved valine residue of C. elegans SRC-1, SRC-2, and human FRK. (B) Representative DIC images of
src-2(Val170Ile) mutant. (C) Quantification of brood size of wild type and missense mutants of
src-1 and
src-2. (D) Percent embryonic viability after feeding control or
src-1(RNAi) in wild-type, and missense mutants of
src-1 and
src-2. (E-H) Representative DIC images of wild type and defective larvae and embryos in missense mutants or following
src-1(RNAi). (I) Schematic outlining the 4D embryonic developmental imaging regime. (J) Plot highlights embryonic lethality data captured by 10x whole well-scan, following overnight imaging regime. (K) Schematic and images show initial patterning of neuronal precursors in wild-type control embryos and after
src-1(RNAi) in control and
src-2(Val170Ile) mutant embryos. Green asterisks highlight patterning of neurons into four quadrants (expected), and red asterisks highlight abnormal neuronal number and/or position. (L) Images show epidermal patterning from the dorsal surface in wild-type control embryos, and after
src-1(RNAi) in control and
src-2(Val170Ile) mutant embryos. Green arrow points to uniform row of seam cells along the lateral surface in control embryo, whereas red arrow indicates disorganized population of seam cells. (M) Images depict time series during enclosure and elongation (controls) and arrest at enclosure or protrusive rupture (yellow arrow) at enclosure in control or
src-1(RNAi) embryos (respectively). Epidermis shown in green, and neurons shown in red. Time is relative to initial red marker expression. (N) Graph quantifies the relative frequency of embryonic phenotypes observed in all tested conditions. Asterisks indicate P<0.05 by one tailed chi-square test in (M). P-values: * = 0.0184 (C); **** <0.0001 (C-D) (one-way ANOVA test). Asterisks indicate P<0.05 by one tailed chi-square test (N). Scale bars are indicated in each graphic.