Fig. 2. Expression of
ceh-63prom::gfp in UL2650. GFP was expressed from
ceh-63prom::gfp (pUL#JRH10H1) in a single nerve cell with its cell body in the tail ganglion and process extending anteriorly to the nerve ring. (A) DIC image of a mid larval stage. (B) Corresponding GFP fluorescence. (C) This cell was confirmed to be the single interneuron DVC by DIC microscopy, the GFP fluorescence superimposed. (D) The axon of DVC forms a ring structure, following round the circumpharyngeal nerve ring, in the head region, as revealed by confocal microscopy. (E, F) Expression was not seen in young embryos and starts in comma stage embryos. (G) GFP also occurs in the uterus in young adult hermaphrodites. (H) The DVC morphology was determined previously from reconstructions of serial electron microscopy sections (White et al., 1986; adapted from WormAtlas). The position in the tail of the cell body of DVC, with respect to those of neurons DVA and DVB, is depicted. The DVC axon in the nerve ring was seen as smooth and non-branching, with a small gap. Bars represent 25 um in A, B and G, 10 um in C, E and F,
and16 um in D.