Figure 1. In
dhc-1;
mel-28 double mutants, proximal oocytes often fail to mature: (A) Immunolocalizations of phosphorylated histone H3 and DAO-5 in proximal gonads captured using structured illumination. The dotted line outlines the proximal gonad, and the -1 oocyte is the right-most oocyte in all panels. In the merged images, blue indicates DAPI staining, red indicates phosphorylated histone H3, and green represents DAO-5. Arrowheads indicate DAO-5-positive nuclei in the merged images. Scale bar = 10 μM. (B) Frequency of DAO-5-positive nuclei in the four most proximal oocytes in each strain. (C) Frequency of nuclei with phosphorylated histone H3 in the four most proximal oocytes in each strain. We used a Fisher's Exact test to compare each mutant with the wild-type at the equivalent oocyte position. *=p<0.05, ** =p<0.01, ***=p<0.001. (D) The number of oocytes scored at each position for each strain.