Figure 1. DEC inhibits C. elegans motility independent of TRP-2. A, B: High concentration of DEC causes flaccid paralysis in C. elegans. Still images of N2 worms thrashing (A) and paralyzed (B) in the presence of 1mM DEC. C, E & G:Show time-dependent effects of high concentrations of DEC on the locomotory speed of N2,
bus-12(
e2977), and
trp-2(
sy691). Worms treated with 1mM and 3mM DEC undergo paralysis immediately after treatment and recover completely in 40 minutes. N=15, 2-way ANOVA, p<0.001. D, F & H: Show concentration-response plots for the motility of N2,
bus-12(
e2977), and
trp-2(
sy691) in the presence of DEC, measured after 0.5 minutes post-treatment. The IC50 values for all strains were not significantly different from each other. N=15, 2-way ANOVA, p>0.05.