Figure 1. The
ut9 allele is an insertion in the
drl-1 locus that results in a severe reduction in vitellogenin expression and slowed development:A) Representative overlaid DIC and mCherry fluorescence images (scale bar, 200 µm) and B) quantification of mCherry fluorescence (median and interquartile range; ****, P<0.0001, one-way ANOVA) of day 1 adult wild-type,
drl-1(
rhd109), and
flr-3(
ut9) animals expressing the Pvit-3::mCherry vitellogenesis reporter. C) Growth rate (mean +/- SEM, n=3) and D) body size (median and interquartile range; ****, P<0.0001, one-way ANOVA) of wild-type,
drl-1(
rhd109), and
flr-3(
ut9) animals. E) Representative overlaid DIC and mCherry fluorescence images (scale bar, 200 µm), F) quantification of mCherry fluorescence (median and interquartile range; ****, P<0.0001, one-way ANOVA), and G) body size (median and interquartile range; ****, P<0.0001, one-way ANOVA) of F1 cross-progeny derived from Pvit-3::mCherry;
flr-3(
ut9) animals crossed to wild-type,
drl-1(
rhd109), or
flr-3(
ut9) animals. H) A schematic showing the genetic lesions within the
drl-1 locus and the primer pairs used to genotype the
ut9 allele. Amplification of the I) wild-type or J)
ut9 drl-1 alleles by PCR in wild-type,
drl-1(
rhd109), and
flr-3(
ut9) animals using the indicated primer pairs. PCR products were resolved by agarose gel electrophoresis. All strains shown here carry the Pvit-3::mCherry reporter.