Figure 3. Regulation of Beta-Oxidation Gene Expression by
nhr-49 (A) QRT-PCR measurement of
acs-2, F09F9.3, and
ech-1 expression in WT (gray bars) and
nhr-49(
nr2041) animals (blue bars). Expression was measured in all four stages of larval development. Error bars represent standard error of measurement. (B) RNAi of
nhr-49,
acs-2, or
ech-1 resulted in increased Nile Red fat staining. Each image shows 3 or 4 representative worms from a population of L4 animals grown on plates containing RNAi bacteria and Nile Red fat-staining dye. (C)
acs-2::gfp is expressed in many tissues including hypodermis (hyp), intestine (int), body wall muscle (bwm), neurons (neu), and pharynx (pha). ACS-2::GFP localizes to subcellular structures in a pattern similar to what has been reported for mitochondrial proteins. (D) Expression of
acs-2::gfp was lower in
nhr-49(
nr2041) mutant animals. (E) Expression of Pnhr-49::gfp promoter fusion in WT animals revealed that
nhr-49 is expressed in multiple tissues, including hypodermis (hyp), body wall muscle (bwm), pharynx (pha), and intestine (int). The animals shown here are genetically mosaic, harboring the Pnhr-49::gfp construct in only a fraction of total cells. (F) Ectopic expression of
acs-2::gfp in
nhr-49(
nr2041) was sufficient to reduce Nile Red staining to WT levels.