Figure 3. Expression of
lin-48. (A,C,E,G) Nomarski DIC micrographs of L1 larvae. (B,D,F,H) Epi-fluorescent micrographs of the same animals. The animal in A and B bears a transgene containing pTJ1038, whereas the animals in C-H bear transgenes containing pTJ1157 or a mutant derivative (see Fig. 2A). (A,B) LIN-48 tagged with GFP is localized to cell nuclei. Nuclei of F and U are indicated. The location of K and K' is marked; the nuclei of these cells are out of the plane of focus. Expression of LIN-48::GFP transgenes is at low levels, and the F cell is expressing at a slightly lower level than the other cells in this particular male larva. (C,D) Expression pattern of
lin-48::gfp in wild type. The location of the hindgut and the excretory duct cell are indicated with an arrow and an arrowhead, respectively. Expression is observed in the U, F, K and K' hindgut cells, the excretory duct cell and several cells in the head in the larva. Phasmid cell expression does not initiate until late L1 stage. (E,F) Expression pattern of
lin-48::gfp transgenes that include mutations in both
lre1 and
lre2. These mutations disrupt expression in the hindgut cells, and excretory duct expression can be reduced. (G,H) Expression pattern of intact
lin-48::gfp transgenes in an
egl-38(
sy294) mutant animal. The mutant animal fails to express
lin-48::gfp in hindgut cells. Scale bars: in A, 20 um for A,B; in C, 20 um for C-H.