Figure 1. Characterisation of
egl-27(
we3) and
egl-27::gfp expression. (A)
vab-7(
ed6) L1 hermaphrodite is nearly identical to wild type except for a small bulge at the tip of the tail (arrow), where the wild-type tail is sharply pointed. (B)
egl-27(
we3) L1 grown at 15 C. (C-F) Muscle patterning in wt (C,D) and
egl-27(
we3) (E,F) embryos at 1.5-fold stage of development. Muscle cells in D and F are visualised using
hlh-1::gfp, a reporter for the C. elegans MyoD homolog (Krause et al., 1990; K. Dej, S. Xu, and A. Fire personal communication); (C,E) DIC images of the same embryos. In D, two of the four muscle rows are visible in this focal plane. Arrow in F points to a cluster of muscle cells. (G,H) Seam patterning in wt (G) and
egl-27(
we3) (H), visualised using antibody NE2/1B4.14; arrowsin H shows forked and disrupted posterior seam. (I) L1 larvae induced by
egl-27(exon 11) RNAi. (J,K)
egl-27::gfp expression in 1.5-fold embryo (J) and L3 hermaphrodite (K). All somatic cells appear to express the reporter gene. Scale bar in B is for B,I; scale bar in C is for C-H and J.