Figure 2. The Diacetyl Chemoreceptor ODR-10 Is Regulated by AWA Genetic Sex(A) Chemotaxis to diacetyl (1:100) in wild-type and
odr-10(
ky225) hermaphrodites (red) and males (blue). Four independent experiments were performed with a total of n = 113, 183, 96, and 97per group (left to right). Unpaired two-tailed t tests were employed to determine significance; asterisks indicate comparisons with wild-type controls (hermaphrodites: p = 0.044; males: p = 0.42). Error bars indicate SEM.(B) Representative examples of ODR-10::GFP (kyIs53) fluorescence in a wild-type hermaphrodite,wild-type male, AWA-masculinized (Pgpa4D6::
fem-3) hermaphrodite, and AWA-feminized (Pgpa-4D6::
tra-2(IC)) male. All images were acquired using the same illumination intensity andexposure time. White dashes indicate the outline of the body.(C) Quantitation of ODR-10::GFP expression. Fluorescence intensity was binned into four categories,as indicated with the investigator blinded to genotype. Experiments were performed on a total of n = 96, 52, 73, 97, 57, and 52 animals per group (left to right) over the course of at least four independent sessions. Kruskal-Wallis with Dunn's multiple comparisons tests were used to determine significance (***p < 0.001).(D) Diacetyl (1:1,000) chemotaxis in hermaphrodites (red) and males (blue), comparing wild-type adults to
odr-10(
ky225) mutants (
odr-10(-)) and
odr-10(
ky225) mutants carrying Pgpa-4D6::
odr-10(+) (odr-10ON). Four independent experiments were performed for a total of n = 124, 131, 97, 119, 120, and 104 in each group (left to right). Two-way ANOVA with Tukey's multiple comparisons tests were employed to determine significance; asterisks indicate post-test comparisons with
odr-10 mutants (***p < 0.001). Error bars indicate SEM. See also Figure S2.