Fig 6. Expression of
fmo-1,
fmo-4 and
fmo-5 in VD/DD neurons rescues axon pathfinding defects. (A) The percentages of VD/DD axons failingto cross the lateral mid-line are as described in Fig 5A.
unc-5 double mutant genotypes are indicated, and the Punc-25::
fmo-1, Punc-25::
fmo-4, andPunc-25::
fmo-5 transgenes are bracketed. Data for transgenic strains are the combined results from three independently-derived transgenes withsimilar effects. Double asterisks (**) indicate a significant difference between the double mutant and the transgenic strain (p <0.001; Fisher's exacttest). Error bars represent 2x standard error of the proportion. (B, C) Micrographs of mutant and rescued animals. Dorsal is up, anterior to the left. Scalebar represents 5um. The lateral midline of the animal is indicated by the dashed white line. The dorsal nerve cord and ventral nerve cord are indicatedby dotted white lines. (B)
fmo-1(
ok405)
unc-5(
e152) axons often fail to cross the lateral midline (arrow). (C)
fmo-1(
ok405)
unc-5(
e152); Ex(Punc-25::
fmo-1) axons crossed the lateral midline (arrows). (D-E and D'-E') Images are micrographs of L2 animals with transgenic expression of Pfmo-4::gfpand Pfmo-5::
fmo-5::gfp. Dorsal is up and anterior is left. Scale bar: 5um. (D)
fmo-4::gfp is broadly expressed, including in hypodermis and in cells alongthe ventral nerve cord that resemble motor neurons. Expression is not evident in the lateral hypodermal seam cells (asterisks). (D') Enlarged image offmo-4::gfp expression in ventral nerve cord cells (arrows). (E).
fmo-5::gfp is expressed strongly in the gut (asterisks), as well as in cells along the ventralnerve cord. (arrow) (E') Enlarged image of
fmo-5::gfp expression in ventral nerve cord cells (arrows).