Figure 3. Developmental profile of
ftt-1 and
ftt-2 ex- pression patterns. Total RNA samples from embryos (lane 1), L1 larvae (lane 2), L2/L3 larvae (lane 3), L4 lar- vae (lane 4) and adults (lane 5) were isolated, and 10 mg of each RNA sample was separated on an agarose gel and sized according to RNA molecular mass markers (BRL). The RNA panel hybridized sequentially with the
ftt-1 cDNA probe (A), the
ftt-2 cDNA probe (B), and 28S rRNA probe (C). The representative autoradio- graphs in A to C were scanned densitometrically across lanes to quantitatively analyze stage specific changes in individual transcripts. These results were converted into graphic form after normalizing the data for sample load- ing variations using the 28 S rRNA marker. The lowest level of each transcript was set to an arbitrary value of 1 in order to optimize comparisons of a single transcript between the staged worms. Direct comparisons cannot be made between different transcripts, since the relative abundance scales are not comparable.