Figure 1: Mapping and location of otDf575 and otDf642. (A,C) Sequence of left arm of LGX, with region from 296,000 to 442,666 enlarged (panel C). The deletion breakpoint is at 405,058 and indicated with a red line. Panel C shows aligned Illumina sequencing reads (146,666 bp, from LGX: 296,000-442,666) for
ot642 and
ot575, as displayed in the UCSC genome browser (Kent et al., 2002). A complete absence of coverage, indicative of a deletion, can be observed for both
ot575 and
ot642. Other strains isolated from the same mutant screen do not show this deletion. (B)
ot642 mapping plot. While
ot575 was mapped by variant discovery mapping (VDM) through crossing with N2 wildtype animals (Method described in Minevich et al., 2014),
ot642 was mapped by WGS-SNP through crossing with Hawaiian polymorphic mapping strain and results are shown in a mapping plot, as previously described (Minevich et al., 2014).