Figure 6.
cacn-1 RNAi reduces LIT-1::GFP expression. LIT-1::GFP animals were grown on E. coli HT115(DE3) carrying (A, B) control RNAi or (C, D)
cacn-1 RNAi vector. Asterisks indicate L4 developing vulva. 3-dimensional representations of GFP expression with peak height corresponding to maximum intensity of fluorescent reporter in the uterus and vulva of (E) control RNAi or (F)
cacn-1 RNAi treated animals. (G) Western blots of extractsfrom control RNAi or
cacn-1 RNAi-treated LIT-1::GFP animals were probed with anti-GFP (LIT-1::GFP) and anti-Actin antibodies. Scale bar is 25 um.