Figure 2. C. elegans Neuroligin Localizes Both Pre- and Postsynaptically in Neurons. (A and B) NLG-1::YFP localizes to pre- and post-synaptic regions of DA9 motor neurons, but is more prominent in the postsynaptic region.(C-E) NLG-1::YFP colocalizes with the synaptic vesicle marker mCherry::RAB-3 in DA9. (C) NLG- 1::YFP. (D) mCherry::RAB-3. (E) Merge. Yellow arrowheads indicate colocalizing puncta, white arrows indicate RAB-3-only puncta. In (A)-(E), DA9 expression was directed by the
mig-13 promoter. (F and G) NLG-1 localizes to pre- and postsynaptic regions of AVE interneurons, labeled using the
opt-3 promoter. P, pharynx.(H and I) NLG-1 localizes to pre- and postsynaptic regions of VA motor neurons, labeled using the
unc-4 promoter. Known synaptic domains (White et al., 1986) are indicated in schematic diagrams (A, F, and H). Anterior is at left in all panels. Scale bars, 10 um.