FIGURE 5: Immunolocalization of CPNA-1 in the null background of other Pat genes. In late-stage, wild-type embryos, CPNA-1 is organized into bands of dense bodies and M-lines in the muscle cell quadrants (A; arrowheads). In null
pat-3 embryos, however, CPNA-1 is mislocalized into large aggregates within body-wall muscle cells (B; arrows). Similarly, large accumulations of CPNA-1 are also seen in null embryos for
pat-4 (C) and
pat-6 (D; arrows), although in some
pat-6 embryos, CPNA-1 mislocalized to a lesser extent than for
pat-3 and
pat-4 embryos (E; arrow). In arrested twofold embryos lacking MYO-3, however, CPNA-1 is organized properly into bands of dense bodies and M-lines (F), as it is in wild-type embryos (A, arrowheads). Bar, 2 um.