Figure 3. GFP expression pattern driven by DNA sequences encompassing the predicted modules. (A) C01B7.1::gfp expression in the pharyngeal muscle. (B) C10G11.7::gfp expression in neurons. F27D4.2::gfp expression in pharyngeal muscle (C) and body wall muscles (D, arrow). F45D3.2::gfp expression in the body wall muscle (E, arrows) and neurons (F). T28B8.1::gfp expression in the pharyngeal muscle (G), vulva muscle (H, arrow), and neurons (I). W06H8.6::gfp expression in the body wall muscle (J), vulva muscle (K), pharyngeal muscle (L), and H cells (data not shown). (M) A merge of DIC image and fluorescent image taken for the same worm showing W06H8.6::dpes10::gfp expression in the pharyngeal muscle cells. (N) K10G6.3::gfp expression in the pharyngeal muscle (arrow) and neurons.
W06H8.6::gfp expression in the body wall muscle (J), vulva muscle (K), pharyngeal muscle (L), and H cells (data not shown). (M) A merge of DIC image and fluorescent image taken for the same worm showing W06H8.6::dpes10::gfp expression in the pharyngeal muscle cells.
C29F5.1 was predicted to express in muscle. The reporter construct was observed in body wall, vulval, and anal but not pharyngeal muscle in all stages.
(B) Localization of UNC-96::GFP. Top, body wall muscle; bottom, pharyngeal muscle. In body wall muscles, UNC-96::GFP is located at M-lines, like the antibodies, but, in addition, is also located at dense bodies. In pharyngeal muscles, UNC-96::GFP is localized similarly to the localization of UNC-96 antibodies.