Fig. 3. GFP expression of
ceh-63 recombineered reporter gene fusions. (A, B, C) The recombineered fosmid fUL#HF003.1, with the
ceh-63 coding region replaced by gfp, drove GFP expression in DVC and in the uterus (open triangle). (D, E, F) The gfp reporter inserted immediately after the start codon of
ceh-63 in fosmid fUL#HF001.1, was also expressed inDVC and the uterus (open triangles), but the GFP signal was low and nuclear-localized. Expression of this N-terminal fusion was not observed in the head even with long exposures.(G, H, I) For fUL#HF002.1, with gfp inserted immediately before the stop codon of
ceh-63, low levels of GFP were again observed in DVC and the uterus, at a similar level to that of the N-terminal fusion, but GFP was also observed in a few other cells in both the head and tail. Images presented are for strains: UL3025 (A, B, C); UL3015 (D, E, F); UL3234 (G, H, I). Exposure times were 1.5 seconds, apart from the 3 second exposure of panel D. Bars represent 25 um.