Fig 2. RAB-10 functions cell-autonomously in the PVD neuron. (A, B) The dendritic morphogenesisdefect in
rab-10 (
ok1494) mutant animals was rescued by PVD-specific expression of GFP::RAB-10 driven from a multicopy extrachromosomal array. PVD morphology was visualized using the PVD>gfp marker strain wdIs51. The inset images are enlarged views (2.5 fold) of the regions indicated by the boxes. Both images are maximum intensity projections of z-stacks. Scale bar, 50 um. (C) Quantification of the cell-autonomous rescue of dendritic morphogenesis defect by cell-specific expression of wild type, GDP-locked, and GTP-locked forms of RAB-10. For each construct, at least two
rab-10 (
ok1494) lines carrying arrays expressing
ser2prom3> gfp::
rab-10 WT, GDP-locked (T23N), and GTP-locked (Q68L) forms were generated. Animals were categorized based on their PVD dendrite morphology. At least 29 animals at the L4 or young adult stage were scored for each line. Partial PVD rescued animals contained one or more full menorahs in the proximal region. Full PVD rescued animals contained PVD dendritic arbors that were indistinguishable from wild type animals. (D) Colocalization of GFP::RAB-10 and mCHERRY::FAPP1-PH in the PVD. Magenta, mCHERRY::FAPP1-PH; green, GFP::RAB-10. (E) Colocalization between GFP::RAB-10 and mCHERRY::RAB-5 in PVD. Magenta, mCHERRY::RAB-5; green, GFP::RAB-10. r-value reports mean Pearson's correlation coefficient +- SEM for colocalization of GFP::RAB-10 and mCHERRY::FAPP1-PH, and GFP::RAB-10 and mCHERRY::RAB-5. At least 15 animals at the L4 or young adult stage were quantified for each group of colocalization analysis. The inset images are enlarged views (2 fold) of the regions indicated by the boxes. Arrows indicate vesicles co-labeled by both GFP and mCherry reporters. Error bars report +- SEM. Scale bar, 10um.