Figure 1. The
gls-1 Genomic Locus and Gene Products. (A) Genetic map position.
gls-1 is a downstream gene in an operon with
dhfr-1 on chromosome (LG) I. The deficiency nDf23 removes genes in the approximate interval of +2 to +4 including
gls-1. Exons, boxes. (B)
gls-1 transcripts and deletion mutants.
gls-1 mRNAs are SL2 spliced and vary slightly in their coding potential in the first exon by 6 nts (not shown). Coding exons are shown in boxes connected by thin lines representing introns. The encoded GLD-3 interaction domain is indicated. Extent of the 1657 bp
gls-1(
ef8) and 1253 bp
gls-1(
ef4) deletions is indicated by gaps. (C) GLS-1 protein structure. Fragments for antibody (ab) generation, the serine/threonine/asparagine-rich (STN), the proline/serine-rich (PS) and the minimal GLD-3 interaction region are outlined. GLS-1 conservation among three Caenorhabditis species is given in percent. brig, briggsae; rem, remanei. (D-E) Immunoblots of protein extracts prepared from indicated genotypes.
glp-1(
q224ts) and
glp-4(
bn2ts) animals with essentially no germ line raised at 25 C. His-tagged recombinant GLS-1 (in vitro) is given as a size reference. GLS-1 was detected in (D) with the central polyclonal antibody and in (E) with the C-terminal monoclonal antibody. A truncated GLS-1 form (arrowhead) of the expected size is present in
gls-1(
ef4) mutant animals. Asterisks mark non-specific background bands, which serve as loading controls (150 worms each lane).