Figure 8 LIN-41 and the OMA proteins regulatespn-4 expression in an antagonistic fashion.(A-F) The expression of
spn-4 was analyzed indissected gonads from wild type (strainDG4158),
lin-41 null (from strain DG4176),and
oma-1;
oma-2 null (from strain DG4239)backgrounds. SPN-4::GFP::3xFLAG expressionwas detected by indirect immunofluorescencein dissected and fixed gonads stained withanti-FLAG antibody. DNA was detected withDAPI. (G) DIC and (H) GFP fluorescence imagesof a living adult hermaphrodite showing anabsence of SPN-4::GFP::3xFLAG expression inproximal oocytes in a
lin-41(
n2914);
oma-1(
zu405te33);
oma-2(
te51) triple null mutant(from strain DG4313). SPN-4::GFP::3xFLAG expression is unaffected in the distal proliferativezone in this genetic background, but this is notshown in this image (T. Tsukamoto and D.Greenstein, unpublished results). All strainsshown contain a
spn-4(
spn-4::gfp::3xflag) edit,which is (A-D)
spn-4(
tn1699) or (E-H)
spn-4(
tn1718). The tight linkage of
spn-4 and
oma2 necessitated independent targeting of
spn-4.
spn-4(
tn1718) and
spn-4(
tn1699) are the sameat the DNA sequence level and exhibit identicalexpression patterns.
oma-1(RNAi);
oma-2(RNAi)prevents expression of
spn-4(
tn1699) (Figure S2in File S4; n = 31). Bar, 50 um.