Figure 1.
kin-3(RNAi) suppresses embryonic lethality and abnormal centrosome behavior in
sur-6(
or550) mutant embryos:Genetic analysis of
sur-6(
or550) mutants at the semi-restrictive temperature (21°C, *** p<0.001) (B) Quantification of mitotic spindle formation (stacked bars) during the second cell cycle in temperature-sensitive
sur-6(
or550) mutants. At 22.5°C,
sur-6(
or550) embryos often display defective centrosome duplication, with monopolar (dark gray) or asymmetric bipolar (light gray) spindles during the second mitosis.
kin-3(RNAi) increases the percentage of symmetric bipolar spindles (green) in
sur-6(
or550) embryos. n is the number of blastomeres scored. The bottom panel illustrates representative embryos expressing mCherry::tubulin, with symmetric bipolar (green arrows), asymmetric bipolar (light gray arrows), and monopolar spindles (dark gray arrowheads) at the second mitosis in
sur-6(
or550) mutants, indicative of defective centrosome assembly during the first cell cycle. (C) % Embryonic lethality of
sur-6(
or550) mutant animals at 22.5°C. Data are presented as mean±s.d. n is the number of progeny scored. (*** p<0.0001)