Figure 6. Reporter gene analysis of cis-regulatory elements of the
unc-122 and
cof-2 genes. A,
unc-122 constructs used to generate transgenic lines. Black bars indicate genomic sequence (5' and 3' of the gene); the white box indicates the heterologous
unc-54 3'UTR. Expression pattern and rescue of the
unc-122 mutant phenotype is indicated (expression is shown in C). For localization of presumptive cis-regulatory regions driving expression in muscle, see Figure 5. GFP and HA epitope coding sequences are not drawn to scale. Antibody staining to detect the HA-tagged protein is shown in Figure 8 in the context of subcellular localization of UNC-122. B,
cof-2 promoter construct. The promoter contains cis-regulatory elements for expression in coelomocytes and muscles as shown in D-G. C-G, Transgenic worms expressing gfp reporter gene constructs. Stars indicate coelomocytes; arrows indicate body wall muscles in E and enteric muscle in all other panels. Most body wall muscles are not visible in D because of mosaicism. C,
unc-122prom::gfp (the 800 bp promoter fusion from A). D-G,
cof-2prom::gfp.