Figure 3. TMEM-135 distribution in C. elegans. Images in A, B and C were obtained using
tmem-135 promoter-driven GFP to assess
tmem-135 tissue expression.
tmem-135 was found to be ubiquitously expressed at all stages (A, B, C; data not shown). Apparent differences in expression pattern reflect the mosaic distribution of the transgene. (A) Shows
tmem-135 expression pattern in the pretzel embryo. (B) Shows
tmem-135 expression pattern in a L1 larva. (C) Shows
tmem-135 expression pattern in an adult worm. Images in D, E and F were obtained using a GFP-tagged full-length TMEM135 to observe TMEM135 sub-cellular localization. Panels D, E and F reveal that TMEM135 localizes to rounded sub-cellular organelles of 0.2-0.5 microns in L1 larvae, which are particularly abundant in the intestine.